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KMID : 0545119990090040514
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Journal of Microbiology and Biotechnology
1999 Volume.9 No. 4 p.514 ~ p.517
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Hydrolysis of Empty Fruit Bunch of Oil Palm Using Cellulolytic Enzymes from Aspergillus terreus IMl 28243
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KADER, JALIL
KRISHNASAMY, GETHA/MOHTAR, WAN/OMAR, OTHMAN
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Abstract
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Hydrolysis of EFB (empty fruit bunch) derived from oil palm was studied using crude enzyme from Aspergillus terreus IM1 282743 along with commercial enzymes from Trichoderma reesei and Aspergillus niger Hydrolysis at 40℃ and 50℃ with α-cellulose or EFB gave significantly lower yield when commercial enzymes of T. reesei and A. niger were used and the hydrolysis time extended beyond 10 h. After 24 h of hydrolysis at 40℃ and 50℃, the filter paper activity (Fpase) from A. terreus retained as much activity as A. niger and it was significantly higher than T reesei. Glucose concentration of 0.25% and 0.5% caused significant inhibition in the crude enzyme, but in regards to the commercial enzymes it only showed a slight effect. Crude enzymes from A. terreus could produce the highest reducing sugars when compared to commercial enzymes from T reesei or A. niger. Nevertheless, low yield of sugar was observed for EFB for all treatments.
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